الملخص

In this study (10) local isolates were identified Clostridium beijerinckii from (100) samples from hard residues of heavy water unit filtration from Ibin Sina hospital in Mosul city, depending on biochemical tests . It has been found that two local isolates (4 & 6) are identical to standard C. beijerinckii in producing butanol. In order to compare the production ability of butanol of the two isolates (4 & 6) with standard isolate Clostridium acetobutylicumATCC824 the bacteria were exposed heat shocks (25, 50, 100 and 150), and when the extracts were analyzed by HPLC we found the highest production of butanol was (1450) from local isolate number (6) after (150) heat shocks, and (1244) from standard isolate C. acetobutylicum ATCC824 and (890) from local isolate number (4). It was concluded that the two isolates (4 & 6) produce butanol after they have been exposed to the heat shocks.