الملخص

The contains an introduction about folic acid (Pteroyl-L-Glutamic acid), as it is one of vitamin B group and plys amajor role in the vital functions of the metabolism cells, such as DNA replication, The aocesses of repairing and synthesis of nucleotides and amino acid, And composition of red blood cells in the bone marrow the rapeutically folic acid provents the probability of birth defects and improves the performace of the memory. It also helps in heart diseases treatinent by controlling homocystine levels in blood. The shortage of folic acid affects the stability of DNA and may lead to increasing the risk of colon and rectal concer infection and defects in the neural tube.The deals with apresentation of the methods thatare used for estimating folic acid in pharmaceutical and foods products. They includes spectroscopic electronic and chromatographic methods. As for the spectroscopic methods, They based on using multiple reagents for estimation under suitable conditions, Through applyin either oxidative coupling organic reactions by interacting with the following systems (Phenothiazine-sodium metapyriodate in acidic media, Ortho-aminophynol-potassium iodate in acidic media, Phenoxazine-sodium metapyriodate in acidic media), or using diazotizition coupling reactions for the direct determination of folic acid through its during his diazotizition and coupling, or resorcinol reagent to form an azo dye product or indirect during diazotizition the fission of the reductive folic acid (Para – amino benzoyl glutamic acid) and its coupling with reagents imino di benzyl and 3-amino phenol detectors, And the two azo dyes at 580 and 460 nm respectively.