Abstract
The research included the description of a simple indirect spectrophotometric method for the determination of pyridoxine hydrochloride, which depended on the oxidation of pyridoxine with an amount of N-bromosuccinimide in the presence of hydrochloric acid and the excess amount of it oxidized the dye Eriochrome black-T, and the absorption was measured at 517 nm, and the molar absorption coefficient was 8 × 103 L / mol. cm, indicating the sensitivity of the method. And compliance with Beer's law for concentrations ranged from (2-14) micrograms. ML-1, the method was characterized by good accuracy and compatibility, with a recoverability of 99.07%. While the relative standard deviation was less than 0.63.The method was applied to pharmaceutical preparations of pyridoxine hydrochloride. So, what have been mentioned above represent clearly the importance of doing such researches in this simple and sensitive one. Researchers within the field of analytical chemistry can follow the same procedures with different dyes .ِ Also, other pharmaceutical preparations ( of local and international sources ) can be worked on holding a comparative study. So, things should be in this direction to reach the best results with low costs.