Abstract
Background Due to the effect of herbicide on the soil, which changes the natural fluorescence, which prevents the growth of bacteria that fix nitrogen and an imbalance, occurs between the organisms that live in the soil. Harmful organisms present in the soil increase until they become a pest. This study came. Methodology Studying the effects of glyphosate on nitrogen-fixing bacteria like Azospirillum normally includes a combination of laboratory and field trials. An overarching framework for conducting such studies is outlined below. Results The average number of Azospirillum cells at each concentration and in the control group shown by the mean values. There were more total counts of Azospirillum in the control group, suggesting a higher mean (8200.00) without glyphosate. There appears to be a dose-dependent effect of glyphosate on Azospirillum count, with the mean values decreasing as the concentration of glyphosate rises from 5% to 20%. The p-values give us an idea of how significant the results are. A p-value of 0.00001 for the 5% concentration level implies an extremely low likelihood of achieving the observed results if there were no meaningful effect.