Abstract
The objective of this study was phenotypic detection of biofilm formation by S.aureus and P.aeruginosa isolated from different clinical specimens using tube method. The results showed that (37%) and (19%) of S.aureus isolates have moderate and weak ability to form a biofilm , respectively, whereas (44%) of S.aureus isolates were unable to form biofilm using this method. On the other hand the ability of P.aeruginosa isolates to form biofilm using tube method was varied between strong, which represent the higher percentage (50%), to moderate and weak which represent (30%) and (10%) respectively whereas only (10%) of P.aeruginosa isolates where unable to form a biofilm using this method..