Evaluation of Genetic Diversity among Eight Tetraploid Wheat Varieties Using Random Amplified Polymorphic DNA (RAPD) Markers
College Of Basic Education Research Journal,
2013, Volume 12, Issue 4, Pages 969-980
AbstractThe Random amplified polymorphic DNA (RAPD) markers are used to determine the genetic diversity among eight varieties of tetraploid wheat’s (Um-rabia 3, Leeds, Azeghar 1, Acsad65, Buhoth 7, Doma 1, Korfela, Um-rabia 5). The total number of the amplified bands (141 bands) are obtained, 101 of which were polymorphic, the DNA fragment size are ranged between 150 - 1650 bp, the polymorphism of RAPD markers are high (70.18%) and adequate to discriminate each variety.
The similarity matrix among wheat varieties for different similarity matrices coefficients (Nei&Li , Jaccard's, and Simple Matching (SM)) are found, the similarity value ranged between 36%-83% . The comparison among similarity coefficients using Mantel test shows that the correlation between Nei & Li and Jaccard similarity matrices was highly significant 0.99. Also, the dendrogram that constructed among wheat varieties using (UPGMA) analysis gives the lowest genetic distance between Azeghar 1 and Doma 1 varieties and the highest between Leeds and Acsad65 for both Nei & Li and Jaccard analysis , while for SM analysis the highest genetic distance was found between Um-rabia 3 and Azeghar 1 and the lowest distance was between Azeghar 1 and Doma1.
The result obtained from the current study reveals that the analysis of RAPD markers using Nei&Li and Jaccard similarity matrices are more informative than SM similarity coefficients to determined the genetic relationships among varieties and confirmed the potential of using RAPD markers to estimate the genetic diversity among wheat’s varieties.
Cited from PH. D. Thesis of the first researcher.
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