Isolation and Diagnosis of Some Species of Corynebacterium from Soil Samples
College Of Basic Education Researches Journal,
2013, Volume 12, Issue 4, Pages 941-956
AbstractThe study included isolation and diagnosis of some species of Corynebacterium from soil samples which were taken from different areas. The isolated bacteria were tested according to their ability to produce glutamic acid infermentative media which were suppliedbyraw material like(apple peels ,whey ,decomposed potato and date syrup)separately. Theproductivity was estimated by standard ninhydrin method, and High Performance Liquid Chromatography (HPLC) and Thin Layer Chromatography (TLC) techniques. The results showed that all species of Corynebacterium have the ability to grow and produce glutamic acid with high rate in fermentative media supplemented with date syrup 37.41%, thenfermentative media supplemented with apple peels 28.45%, and fermentative media supplemented with whey17.25%,whilefermentative media supplemented withdecomposed potato was recorded less rate in productivity16.86%. Finally, the study showed that C. callunae was the best isolated bacteria in producing glutamic acid in the fermentative medium supplemented with raw material.
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