Changing in Collagen Protein Due to Aging
College Of Basic Education Research Journal,
2013, Volume 12, Issue 1, Pages 716-732
AbstractThis study involved the elucidation of aging effects on the some biochemical characters of collagen that has been isolated from rabbit's lungs extract. Twenty-one albino rabbits aged between 1month-3.5 years, classified into 7 groups with 3 rabbits in each.Our results showed biochemical changes in collagens content which increased in rabbit's lung extractfrom 0.012g to 0.079gwhile the percentage of total collagen dry weight/lung dry weight increased in the group 1.5 years 0.094 compared with 1 month group 0.062 then return to decrease in 3.5 years group 0.062 compared to the 1.5 years group.This study also revealed structural changes of purified collagen from rabbits lung extracts with aging,purified collagen showed an increase of carbohydrates content and a decrease in fluorescents absorbance intensity.After using fluorescent spectrophotometric technique,while IR-technique didn't give us any differences in theimportant groups of collagen with aging, but it gave us a finger print similar to that of collagen type I.
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