College Of Basic Education Research Journal

Abstract

The study included the isolation and identification of the bacteria causing wound infection in two types of patients (diabetic and non diabetic).
(60) samples were collected from wound infection, (18) samples from diabetic patients (30%) and (42) were from non diabetic patients (70%). These samples represented different ages and both genders. The results show that all samples of diabetic patients was positive growth (100%), (32) isolate were obtained from all samples, include (12) isolate of Gram negative bacilli (37.5%) and (20) isolate of Gram positive cooci (62.5%). Non-diabetic patients samples give (38) samples (90.47%) bacterial growth. (95) isolate were obtaind from this samples. (52) isolate (54.73%) of Gram positive cooci and (43) isolate of Gram negative bacilli (45.26%) and (4) samples were negative for bacterial growth (9.52%).
The results also show that the highest percentage of these strains are Staphylococcus aureus (40.62%) from diabetic patients and (35.79%) from non-diabetic patients. While the lowest percentage shows in Proteus spp. and Streptocoous spp. (6.25% and 3.13%) in diabetic patients and (4.21% and 3.16%) in non-diabetic patients, respectively.
The study also includes assessment of the antibacterial activity of Nigella sativa oil against the isolates, using disk diffusion method compared with antibiotics used in this study.
The results showed that Nigella sativa oil have a good antibacterial activily on all bacterial types. The oil give good inhibitory effects on Staph. aureus and Streptococcus spp. Whose effective concentration reached (0.033) v/v. also the oil were more effective than the control antibiotics.