This study was performed to separate and identify a number of amino acids present in the bark, sapwood, and heartwood of Ceratonia siliqua L growing in Mosul, seven of the amino acids were identified in the bark, eight in the sapwood, and seven in the heartwood. In general, the number of identified acids in the carob understudy is eight, which are: Aspartic Acid, Phenylalanine Acid, Glumatic Acid, Lysine Acid, Glycine Acid, Tryptophan Acid, Lucien Acid, and Serine Acid. The highest quantities were in the sapwood and the lowest was in the bark. These results are consistent with the results of the study samples (bark, sapwood, and heartwood) of carob trees. The bark, sapwood, and heartwood are similar in terms of amino acids with the exception of serine, which did not appear in both bark and heartwood, and glutamic acid did not appear in sapwood, the presence of this large number of amino acids in carob wood may be due to the ability of carob trees to fix nitrogen in root nodes.