Keywords : Plant

Tissue Cultivation of Plant Raphanus sativus L. and Identification of Raphaiol Alkaloid of Extraction of The Seeds, Explants, Callus and produced Plants from tissue Cultivation

Taghreed Nawaf Ahmed

College Of Basic Education Research Journal, 2013, Volume 12, Issue 2, Pages 691-706

The present study managed the initiation of callus from leaves, stems and roots of the sterilized Raphanus sativus L. Seeds using the solid MS supplied with various concentrations of growth regulators (BA, NAA). The root parts showed the highest percentage of 100% in the presence of 1.0 mg/liter of NAA and BA. Then the stem parts in which the callus initiation estimated at 98%, and the leaves parts with 88% percentage initiating which is done in the same medium. The callus of the stem and the leaves of Raphanus sativus L. has showed a high ability of regeneration of shoots in the same medium.
Moreover, the results of isolation and identification of Raphaiol alkaloid from the seeds , the plant parts (leaves, stems, and roots) and the produced callus from them and the leaves of the plants produced from callus by using the thin layer chromatography showed an isolated spot from each tested sample .These samples showed perfect conformity with the rate flow (Rf) and perfect and approximate conformity with the rates flow of the standard Raphaiol alkaloids spot (the control sample amounted to)=(0.59).

The Plant Callus of Thyme Thymus vulgaris L.

Abdullah N; Taghrid N. Ahmed; Zena Y. Kasem

College Of Basic Education Research Journal, 2012, Volume 11, Issue 4, Pages 623-636

The present study managed to initiate the callus of the plant parts (leaves, stems and roots) from the sterilized complete plants of the Thymus vulgaris L. in MS medium which was supplied with various of growth regulator (BA and NAA) and (Kin and 2,4-D) which reached production on percentage 100% in the medium which included BA and NAA in all parts (leaves, stems and roots) but in various periods, the best medium is MS included 1.0 mg/L of BA and NAA 0.5 mg/L. The best part to initiate callus in short periode (7-10) days were leaves explant, next the stems part (7-14) day, and root in long periode (5-30) days.
The callus of the leaves, stems and roots of Thymus vulgaris L. had a high ability of forming green branches in the used planting medium which consisted in the same concentration 1.0 mg/L of BA and 0.5 mg/L of NAA. This is called one step regeneration, the produced plants of Thymus vulgaris L. from the plant callus were acclimatized in the conditions of the glass house after the success of the enrooting process in free MS medium.

The Effect of Some Aqueous Plant Extracts on Rust-Red Flour and Khapra Beetle

Adnan M. Mohammed; Najem S. K; Aead Y. I

College Of Basic Education Research Journal, 2008, Volume 7, Issue 4, Pages 300-316

The present study deals with the effects of 27 aqueous plant extracts at the concentrations 10, 7.5, 5 and 2.5% of 27 on rust-red fluor beetle , Tribolium castaneumHerbs and khapra beetle Trogoderma granarium Everts . The examined extracts plants represents numbers plants species belonging to twenty families .
In genral mortality percentages was proportionally increased with the extract concentration . The extracts concentration mortality 50 % of treated larvae ( Lc50) were calculated .
Appled extracts were significantly more effective on Khapra larvae weight in relation to rustry _ red flour beetle larvae .